Innovative Cell Technologies is the worldwide sole manufacturer of Accutase and Accumax. Accutase is the gentle replacement for trypsin in all adherent cell culture and particularly important when working with sensitive cell types.
Testing: Accutase has been extensively tested in research and biopharmaceutical laboratories.
Cell Lines: Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Application: Accutase performs exceptionally well in detaching cells for: analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.
Formulation: Accutase has both protease and collagenolytic activity. Each lot of Accutase is individually formulated to meet quality control standards for effective cell detachment. Accutase is supplied in Dulbecco's PBS containing O.5mM EDTA·4Na and phenol red.
Availability: Accutase is available in ready-to-use 100ml bottles.
Testing: Accumax has been extensively tested in research and biopharmaceutical laboratories.
Cell Lines: Accumax has been shown effective in dissociating cell clumps in a variety of cell lines including hybridomas, CHO, BHK, 293, COS and Sf9 cells.
Application: Accumax performs exceptionally well in dissociating cell clumps for cell counting, viral transfection assays, cell sorting, and flow cytometry as well as bioreactor scale-up.
Formulation: Accumax has protease, collagenolytic and DNase activity. Each lot of Accumax is individually formulated to meet quality control standards. Accumax is supplied in Dulbecco's PBS.
Availability: Accumax is available in ready-to-use 100ml bottles.